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Built upon the tremendous success of the NovoCyte® line of flow cytometers, ACEA Biosciences now introduces the most advanced benchtop flow cytometer on the market today, the NovoCyte Quanteon™.
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Take the “Quanteon™” leap and reap the benefits of simplified single cell, high throughput, multiparametric analysis.
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Exquisite sensitivity and 7.2 log dynamic range make the Quanteon™ ideal for the most demanding assays in Flow Cytometry
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“Amazing instrument, it is the future of flow cytometry!” “I have only wonderful things to say about the Novocyte®. It is incredibly easy to use, we get reproducible results, the software is the best one out there, the maintenance is super easy, and ACEA has excellent technical support! Can’t wait to upgrade to the 25-channel instrument!“ – Luz M. Londoño, Halozyme Therapeutics Inc.(review from SelectScience) |
Transformative simplicity, that meets the needs of flow cytometrist’s of every level of experience
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“Software is straightforward, and the software interface is easy to handle” “This (NovoExpress®) software is straightforward, and the software interface is easy to handle. The implemented auto compensation and hierarchic tree structure is a highlight for effective organization of experimental data.” – Matthias Schiemann, Technische Universität München |
Complete walk-away automation with the NovoSampler Q™
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“My best friend in the lab!!!” “I use the NovoCyte® to screen compounds for induction of micronuclei in the TK6 cell line as part of our gene tox strategy. With the NovoSampler® and 96-well format, the assay is extremely robust and the results reproducible to a very high degree. The NovoCyte® is user friendly and creating new applications to support our immune tox strategy has been delightfully easy, even for a novice within the field of immunology.” – Joan Christiansen, H. Lundbeck A/S. (NovoCyte review from SelectScience) |
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The NovoCyte Quanteon™ is a breakthrough in flow cytometry design in that it provides 25 fluorescent channel capability, with light collected against 25 different detectors. Since the detectors have a 7.2 log dynamic range, coupled with advanced auto compensation features, the need to tune each detector has been completely alleviated. You now have the flexibility to choose from 25 fluorescent channels utilizing four lasers, without having to sacrifice the performance in one channel, for another channel.
The fluorescent channels available on the NovoCyte Quanteon™ from ACEA include:
The proprietary optical detection system found inside the NovoCyte Quanteon™ employs a series of 25 Silicon photomultipliers (SiPM), which have superb detection sensitivity, at the photon level. The novel design provides highly discrete management of each fluorescent channel, superior optical detection, and cross-talk between channels is completely minimized. The NovoCyte Quanteon™ also has an automatic optical filter recognition feature, which allows users to efficiently change filter configurations, as needed by the user. The system has a fail-safe hardware feature, which conveniently alerts the user to any improperly arranged filter configurations. This feature mitigates against potential hardware errors during system set-up, which makes it ideal for a multi-user environment.
What is a SiPM?
Silicon photomultipliers (SiPM) are solid-state, silicon-substrate-based, photon-level-sensitive semiconductor devices, with a 7.2 log dynamic range. Consisting of a compact array of avalanche photodiodes operating in unison, the SiPM is a compact detector with photon counting capability. The innovative optics designed into the NovoCyte Quanteon™ incorporates 25 independent SiPM, which collect and process signals for each of its fluorescence channels.
SiPM’s strengths:
Equipped with lasers, optical filters, detectors, and fluidics of the highest quality, the Quanteon™ provides consistent signal detection, with low CV’s. When combined with superior fluidic feedback control mechanisms, the Quanteon™ maintains steady flow rates with remarkable consistency. As shown below, the Quanteon™ provides superior stability across a wide range of sample flow rates and operating conditions, which is a critical requirement for any high end flow cytometer. The NovoCyte Quanteon™ gives you peace of mind, so that you can focus more on your experiments, with the assurance that your Quanteon™ will perform admirably.
Direct Absolute Cell Count Makes Reference Beads Obsolete
On the NovoCyte Quanteon™, a pulse free, highly precise syringe pump is employed to drive your sample, which enables exceptionally accurate absolute counts.
A Fluidic System with Superior Reproducibility & Stability
The fluidic system found on the Quanteon™ is designed to deliver superior performance. When compared to other flow cytometers, the fluidic consistency and stability of the Quanteon™ is unmatched. Other instruments utilizing peristaltic pumps are often plagued with fluidic pulsation, causing inconsistency and inaccuracy in absolute cell counts. |
The NovoCyte Quanteon™ provides enhanced automation capabilities with faster and higher throughput:
The new 1.3.0 version of NovoExpress® software supports the new NovoCyte Quanteon™ flow cytometers, the NovoSampler Q™ autosampler, and the full line of NovoCyte® flow cytometers and NovoSampler’s®.
The NovoExpress® Software is an easy-to-use and highly intuitive software package, which can be employed for both Flow Cytometry data collection and analysis, it provides a powerful range of professional flow cytometry data analysis functionalities, as well as a number of turn-key data analysis functions.
The highlighted features include:
Equipped with 25 fluorescence channels from 4 lasers (405, 488, 561, and 637nm), the Quanteon™ offers tremendous flexibility in panel design. Optimized detector settings eliminate the need for complicated and laborious adjustments, making data acquisition as simple as load-and-go.
Figure 7: Above, we show an 18-color pan-leukocyte immunophenotyping panel on PBMCs using the NovoCyte Quanteon™, adapted from OMIP-024 (Cytometry Part A, 85A:995-998, 2014). This panel was designed to monitor different immune subsets with activation and differentiation markers.
NovoCyte Quanteon’s™ FSC/SSC detection optics and signal processing electronics have been optimized to resolve particles of size down to 0.1 μm. With such superior resolution, platelets, bacteria, and various sub-micron particles can be readily identified and analyzed.
Figure 8: NovoCyte Quanteon™ Flow Cytometer for 0.1~50 µm size particle detection
Cat. No. |
Description |
2010097 |
ACEA NovoCyte Quanteon™ 4025 Flow Cytometer (4 Lasers, 25 FL channels) |
2010099 |
ACEA NovoCyte Quanteon™ 4020 Flow Cytometer (4 Lasers, 20 FL channels) |
2010100 |
ACEA NovoCyte Quanteon™ 4016 Flow Cytometer (4 Lasers, 16 FL channels) |
2010101 |
ACEA NovoCyte Quanteon™ 4000C (4 Lasers, 16~25 FL channels) Custom Build Flow Cytometer |
2010098 |
ACEA NovoCyte Quanteon™ 4025 Flow Cytometer (4 Lasers, 25 FL channels) and NovoSampler® Q Combo |
2010102 |
ACEA NovoCyte Quanteon™ 4020 Flow Cytometer (4 Lasers, 20 FL channels) and NovoSampler® Q Combo |
2010103 |
ACEA NovoCyte Quanteon™ 4016 Flow Cytometer (4 Lasers, 16 FL channels) and NovoSampler® Q Combo |
2010104 |
ACEA NovoCyte Quanteon™ 4000C Custom Build Flow Cytometer and NovoSampler® Q Combo |
2020098 |
ACEA NovoSampler® Q (For NovoCyte Quanteon™ only) |
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